This study was conducted at El-Mattana Agricultural Research Station, Luxor Governorate, Egypt (latitude of 25.17° N and longitude of 32. 33°E) on three sugar crop cycles 2017/18 (plant cane crop), 2018/19 (the first ratoon) and 2019/20 (the second ratoon) to screen sugarcane genotypes for yield, quality and borer resistance, also estimates the relationship among agronomical and pathological traits based on principal components biplot analysis. Results revealed that significant differences among sugarcane genotypes in susceptibility to the lesser sugarcane borer, Chilo agamemnon infestation measured as bored stalk%, bored joints %, girdled stalks%, mean no. holes/joint, mean no. holes/bored joint and mean no. holes/stalk in the three studied seasons. In plant cane and first ratoon seasons the genotype L 62-96 exhibited the highest bored stalk %. While, in the second ratoon, the genotype G.T.54-9 was recorded the highest bored stalk %. Three genotypes (F. 150, M 57-35 and G. 2008-20) were the most tolerant in plant cane season recording the lowest bored stalk%. While, in the 1^st ratoon season, EH88/5-27 and SP72-5181 genotypes were recording lowest bored stalk%. Meanwhile, in the 2^nd ratoon season, SP 81-3250, F. 150, F 161 and G. 2007-61 genotypes were recording lowest bored stalk% respectively.