Two pot experiments were performed in the Experimental Farm, Soils and Water Department, South Valley University, Qena, Egypt during two successive winter seasons (2018/2019 and 2019/2020). The main objective is to evaluate the effect of four addition levels of farmyard manure (FYM) (0, 10, 20 and 30 g/Kg of soil) combined with acid producing bacteria (APB) + molasses, yeast + molasses or their mixture on improving the chemical properties of a calcareous sandy soils and enhancing wheat growth. The results showed that, the results showed that with the increasing added level of FYM the soil pH and CaCO3 content were decreased and the soil EC, OM, total N and available P and K were increased. In addition, plant dry matter, N, P and K uptakes and use efficiencies were increased. Also, the application of APB + molasses, yeast+ molasses or their mixture with (FYM) were more effective than adding FYM alone in the improvement of calcareous soil properties and enhancement of wheat growth. It could be concluded that the best treatment in improving the calcareous sandy soil properties and increasing wheat growth, nutrients uptake and use efficiencies was the treatment of FYM with APB + yeast+ molasses especially when FYM was applied ate level 30 g/kg soil.