Bio-stimulants play a vital role in the sustainable development of horticultural crops. The aim of this study was to assess the effect of synthesis stimulator (salicylic acid) and bio-stimulants (moringa extract, seaweed extract) on growth, yield and quality of Roselle, Hibiscus sabdariffa L., plants. The recorded growth parameter included plant height, number of lateral branches per plant, branch diameter, and leaf area. For yield traits, the calyxes' fresh and dry weight was recorded. Leaf pigments included chlorophyll a, b, and carotenoids. Anthocyanin content in calyxes was measured. The experiment was conducted during 2018 and 2019 seasons, and was designed in completely randomized system. The results showed that application of bio-stimulants showed positive effects on all traits, and the best results were shown under treatment of seaweed, followed by moringa leaves extract. There were positively associations of calyxes yield and its anthocyanin content with other growth traits and photosynthetic pigments. These results highlighted the important role of bio-stimulants, especially seaweed extract, as a bioactive stimulant. Therefore, it is recommended to include seaweed extract in fertilization programs for achieving sustainable production system of such crops.