This study was conducted to investigate the chemical fractionation and mobility of Cu, Cd and Pb in some soil irrigated with sewage water in Qena governorate, Upper Egypt. Sequential extraction results showed that in the treated soil, a major proportion of Cu, Pb, and Cd was associated with poorly crystalline iron oxide bound (PCFeOX), crystalline iron oxide – occluded (CFeOX)  and a major proportion of Cu, Pb and Cd was associated with residual (RES) fractions. Based on relative percent. the results also show that The non-labile Cu, Cd and Pb in the non-irrigated soil partially transformed to the potentially labile Cu due to the irrigation with sewage water Also, a part of the readily labile and potentially labile Cu, Cd and Pb of the sewage water irrigated soils for short time transformed to the non-labile fraction with irrigation with same water for a long time. Higher mobility of metals in the soils irrigated with sewage water for short time represent a potential hazard for the redistribution and translocation of these metals into the food chain and leach to ground water.