Trichinellosis is a globally re-emerging parasitic disease. The available current treatments are
more or less not effective especially for larvae with many drawbacks. Consequently, appropriate
natural treatment is needed. The present study evaluated the in-vitro anthelminthic effects of
Olibanum (OL) extract against Trichinella spiralis adults and larvae. Adults and muscle larvae
were incubated with OL extract at concentrations of 10, 25, 50, 100, & 150μg/ml on culture medium.
The histopathological changes and mortality both stages were assessed by using scanning
electron microscopy (SEM). The OL extract showed significant anthelminthic activity against
adult and larval stages. The maximum and the earliest inhibitory effect were with 150μg/ml
(100% mortality at 36hr incubation) for both stages. However, a concentration of 100μg/ml
showed prolonged incubation time (48hr). Difference between both concentrations was significant
(P≤ 0.5). Besides, T. spiralis adults and larvae incubated with OL extract at concentrations
of 100 &150μg/ml exhibited various histopathological changes in the form of cracks, blebs and
areas of degenerative changes with loss of normal annulations.