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257454

VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES

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Last updated: 25 Dec 2024

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Abstract

Giardiasis and cryptosporidiosis are common protozoa causing gastrointestinal troubles with symptoms
ranged from asymptomatic to long-lasting diarrhea with mal-absorption. The present work was
carried on a total of 96 cases; 62 of them presented with GIT symptoms with or without diarrhea, 50%
of them was previously diagnosed as giardiasis or cryptosporidiosis infected and treated 1-2 months
ago, 11 cases were free from GIT symptoms and 23 cases suffered from chronic debilitating disease as
cancer or renal disease, some of them have previous history of gastro-intestinal disturbances.
The rapid immunochromatographic test (ICT) and copro-ELISA detected G. lamblia and C. parvum
antigens in all fecal samples compared to formol ether concentration and Modified Ziehl-Neelsen
(MZN) staining technique. The sensitivity of G. lamblia in stool samples was 60%, 98.3% & 96.6%
using concentration technique, ICT & ELISA respectively, and specificity was 98.75%, 96.43% &
96.43% respectively. The sensitivity of C. parvum with MZN stain and antigen detection by ICT&
ELISA was 46.2%, 96.4% & 100% respectively. Specificity of the tests in detecting C. parvum was
better than Giardia; 96.4% -100% respectively. All tests have 100% PPV and 80-888% NPV for the
immunological techniques with superiority of ICT over ELISA.

DOI

10.21608/jesp.2022.257454

Keywords

Giardia, Cryptosporidium, ELISA, coproantigen, ICT, MZN, Formol ether concentration

Authors

First Name

ENGY

Last Name

NAHAS

MiddleName

AHMED ABDALLAH

Affiliation

Department of Parasitology, Faculty of Medicine, 6th October University

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Orcid

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First Name

SAYEDA

Last Name

AUFY

MiddleName

M.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

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Orcid

-

First Name

MOUSA

Last Name

ISMAIL

MiddleName

A. M.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

REHAM

Last Name

MOHAMED

MiddleName

KAMAL

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt

Email

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City

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Orcid

-

First Name

DINA

Last Name

EL AKKAD

MiddleName

M. H.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt

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-

City

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Orcid

-

Volume

52

Article Issue

2

Related Issue

36454

Issue Date

2022-08-01

Receive Date

2022-09-01

Publish Date

2022-08-01

Page Start

261

Page End

266

Print ISSN

1110-0583

Online ISSN

2090-2549

Link

https://jesp.journals.ekb.eg/article_257454.html

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https://jesp.journals.ekb.eg/service?article_code=257454

Order

257,454

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES

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Article

Created At

22 Jan 2023