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235822

CRYPTOSPORIDIOSIS: MOLECULAR ANALYSIS, RISK FACTORS AND SEASONAL ABUNDANCE IN IMMUNOCOMPETENT AND IMMUNOCOMPROMISED PATIENTS, KAFRELSHEIKH UNIVERSITY HOSPITALS

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Last updated: 25 Dec 2024

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Abstract

This study detected of the molecular Cryptosporidium species in immunocompetent and immunocompromised
adults with diarrhea attended Kafrelsheikh University Hospitals and to assess
risk factors. Stool samples from 200 immunocompetent & 200 immunocompromised were
examined using acid fast (AF) stain and PCR/restriction fragment length polymorphism (RFLP)
targeting small subunit (SSU) rRNA gene. Cryptosporidium PCR amplified was 15(7.5%) in immunocompetent
and 23 (11.5%) immunocompromised, with significance difference as to age,
presence of mucous, abdominal pain (P<0.05). Cryptosporidium was more common in rural
areas in autumn and winter seasons. Two genotypes were C. hominis and C. parvum, but C.
hominis was 60% in immunocompetent and 82.6% in immunocompromised.

DOI

10.21608/jesp.2022.235822

Keywords

patients, Immunocompetent, Immunocompromized, cryptosporidiosis, SSU gene, RFLP

Authors

First Name

MARWA

Last Name

GHALLAB

MiddleName

M.I.

Affiliation

Departments of Medical Parasitology, Faculty of Medicine, Kafrelsheikh University

Email

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City

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Orcid

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First Name

MOUSA

Last Name

ISMAIL

MiddleName

A. M.

Affiliation

Departments of Medical Parasitology, Faculty of Medicine, Cairo University

Email

-

City

-

Orcid

-

First Name

TAMER

Last Name

HAYDARA

MiddleName

-

Affiliation

Departments of Internal Medicine, Faculty of Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

First Name

SALWA

Last Name

MORSY

MiddleName

M.

Affiliation

Departments of Medical Parasitology, Faculty of Medicine, Cairo University

Email

-

City

-

Orcid

-

Volume

52

Article Issue

1

Related Issue

33960

Issue Date

2022-05-01

Receive Date

2022-05-09

Publish Date

2022-05-01

Page Start

117

Page End

122

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 52_Issue 1_Pages 117-122.pdf

PDF . 821.3kB

Link

https://jesp.journals.ekb.eg/article_235822.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=235822

Order

14

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

CRYPTOSPORIDIOSIS: MOLECULAR ANALYSIS, RISK FACTORS AND SEASONAL ABUNDANCE IN IMMUNOCOMPETENT AND IMMUNOCOMPROMISED PATIENTS, KAFRELSHEIKH UNIVERSITY HOSPITALS

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Article

Created At

22 Jan 2023