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165943

AMELORATIVE AND HEPATOPROTECTIVE EFFECTS OF FISETIN ON ACUTE MURINE TOXOPLASMOSIS

Article

Last updated: 25 Dec 2024

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Abstract

Toxoplasma gondii (T. gondii) protozoan invades any nucleated cell with a special predilection to
reticuloendothelial cells. Liver is severely affected during acute toxoplasmosis. Promisingly, fisetin
(FIS) flavonoid was proved to have antioxidant, anti-inflammatory, anti-cancerous, and anti-protozoal
effects. This study was designed to evaluate the anti-Toxoplasma effect and the hepatoprotective potential
of FIS in vivo. Mice were infected with the virulent RH strain of T. gondii in a dose of
1x103tachyzoites/mouse. Mice were divided into seven equal groups: G1: healthy control, G2: infected
control, G3: Pyrimethamine® (PYR)/sulfadiazine (SDZ)), G4: oral-FIS, G5: oral-FIS+ PYR treated,
G6: intraperitoneal (I.P) FIS, and G7: I.P FIS+PYR treated. Survival rate was estimated, and tachyzoites
were counted in liver impression smears. Liver enzymes and C reactive protein (CRP) were measured
in mice sera. Liver tissues were examined for inflammatory mediators; cyclooxygenase 2 (COX-
2), interleukin (IL) -10, IL-12, IL- , oxidative mediators; inducible nitric oxide synthase (iNOS),
myeloperoxidase (MPO), and malondialdehyde (MDA) FIS showed a moderate effect on parasite survival
and tachyzoites count, with an excellent effect in reducing serum liver enzymes activity and proinflammatory
mediators. Combined (FIS+PYR) showed the best anti-parasitic and hepatoprotective
actions. Its administration I.P. gave significant cure compared to oral route. FIS when given I.P than
oral FIS had a good anti-inflammatory and antioxidant effect, decreased inflammatory cytokines and
improved hepatic pathology of acute toxoplasmosis. Combined FIS/PYR was the best drug regimen.

DOI

10.21608/jesp.2021.165943

Keywords

Toxoplasma gondii, Fisetin, Arginase inhibitor, Oxidative stress, iNOS, Interleukins, Liver enzymes

Authors

First Name

DALIA

Last Name

ELMEHY

MiddleName

A.

Affiliation

Departments of Medical Parasitology,

Email

-

City

-

Orcid

-

First Name

AMINA

Last Name

SALAMA

MiddleName

M.

Affiliation

Departments of Medical Parasitology

Email

-

City

-

Orcid

-

First Name

NEMA

Last Name

SOLIMAN

MiddleName

A.

Affiliation

Departments of Medical Biochemistry

Email

-

City

-

Orcid

-

First Name

REEM

Last Name

ELKHOLY

MiddleName

A.

Affiliation

Departments of Medical Pharmacology

Email

-

City

-

Orcid

-

First Name

DINA

Last Name

TAHOON

MiddleName

M.

Affiliation

Departments of Medical Pharmacology

Email

-

City

-

Orcid

-

First Name

RASHA

Last Name

MADY

MiddleName

F.Department of Medical Parasitology

Affiliation

Department of Medical Parasitology

Email

-

City

-

Orcid

-

First Name

GHADA

Last Name

GAMEA

MiddleName

A.

Affiliation

Departments of Medical Parasitology

Email

-

City

-

Orcid

-

Volume

51

Article Issue

1

Related Issue

24162

Issue Date

2021-04-01

Receive Date

2021-04-23

Publish Date

2021-04-01

Page Start

79

Page End

88

Print ISSN

1110-0583

Online ISSN

2090-2549

Link

https://jesp.journals.ekb.eg/article_165943.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=165943

Order

11

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

AMELORATIVE AND HEPATOPROTECTIVE EFFECTS OF FISETIN ON ACUTE MURINE TOXOPLASMOSIS

Details

Type

Article

Created At

22 Jan 2023