Hydatid cyst (HC) protoscolices somatic antigens (HCPsS-Ag) derived from the HC extracted
from patients, sheep; equine and HC antigen purchased with ready-made diagnostic kits were
evaluated in diagnosis of zoonotic hydatidosis. The sensitivity and specificity of these antigens
in capturing anti-HC-IgG-antibodies (anti-HC-Abs) were measured by ELISA in infected hosts'
sera. Sensitivity of HCPsS-Ag extracted from HC of human origin to diagnose anti-HC-Abs in
naturally patients was 90.0%. The sensitivity of HCPsS-Ag extracted from sheep, equine and
ready-made diagnostic kits (R.M.K.) were (71.4%; 57.1% & 91.9%) respectively. The median
ELISA OD values of positive HC patient samples versus HCPsS-Ag of sheep and equine was
significant (P≤0.05) lower than screened same sera versus HCPsS-Ag of human origin. For
agreement analysis, Kappa values did show significant difference between human sera versus
HC antigen extracted from human or sheep (K= 0.385, P=0.168). Receiver Operating Characteristics
(ROC) analysis showed accepted human diagnosis by using human or sheep originated
antigens. HCPsS-Ag of human origin showed low cross-reaction with Schistosoma mansoni and
Hepatitis C virus, than those of animal origin.