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131060

EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS

Article

Last updated: 25 Dec 2024

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Abstract

Cryptosporidium is recognized globally as a major etiology of persistent and chronic diarrhea
especially in immunocompromised patients and children with significant morbidity and mortality
rates. Moreover, it is one of the most important pathogens causing waterborne outbreaks.
Hence, rapid diagnosis is crucial. In this study, we tried to determine the diagnostic performance
of Rida®Quick Cryptosporidium cassettes versus microscopy of the modified acid-fast
stained smear. Forty fecal samples were collected from immunocompromised patients complaining
of chronic diarrhea. All samples were examined by Rida®Quick Cryptosporidium cassettes
for copro-antigen detection, and microscopically using concentration techniques and
modified acid-fast staining. Cryptosporidium was detected in (45%) of immunocompromised
patients. The sensitivity, specificity, PPV, and NPV of Rida®Quick Cryptosporidium cassettes
were 88.9%, 95.5%, 94.1% & 91.3% respectively with an accuracy of 92.5%. There was no
cross-reactivity with other intestinal parasites. Rida®Quick Cryptosporidium cassettes provide
adequate sensitivity and specificity and give rapid results. Our results recommend its use as an
alternative test in certain situations especially when screening large populations is needed urgently
in a short time, as in outbreaks and epidemiological surveys.

DOI

10.21608/jesp.2020.131060

Keywords

Acid-fast staining, Cryptosporidium, Rapid diagnosis, Rida®quick

Authors

First Name

SHIMAA

Last Name

MOHAMED

MiddleName

REFAEY

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt

Email

moustafad658@yahoo.com

City

-

Orcid

-

First Name

HANAA

Last Name

EL-HADY

MiddleName

AHMED

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt

Email

-

City

-

Orcid

-

First Name

AMAL

Last Name

AHMED

MiddleName

MOSTAFA

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.

Email

-

City

-

Orcid

-

Volume

50

Article Issue

3

Related Issue

19517

Issue Date

2020-12-01

Receive Date

2020-12-19

Publish Date

2020-12-01

Page Start

477

Page End

482

Print ISSN

1110-0583

Online ISSN

2090-2549

Link

https://jesp.journals.ekb.eg/article_131060.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=131060

Order

2

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS

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Article

Created At

22 Jan 2023