The zoonotic intestinal protozoan parasites and Helicobacter pylori) are worldwide distributed
human pathogens. This study evaluated the co-infection between H. pylori and the commonest
intestinal protozoan parasites. A total of 240 stool samples were collected over one year
from patients attending outpatients' clinics of Kafrelsheikh University Hospitals, after verbal
consent for participation. Samples were subjected to microscopic examination directly before
and after formol ether concentration. Samples were examined using merthiolate iodine formalin
(MIF) direct smears to detect G. intestinelis and permanent modified trichrome and modified
acid-fast cold kinyoun's stained smears to detect E. histolytica/dispar and Cryptosporidium spp.
respectively. Screening with immune-chromatographic test (ICT) detected H. pylori infection.
The results showed that H. pylori were in 160/240 (66.7%). Univariate analysis showed significance
association between H. pylori and protozoan causing diarrhea (P = 0.015). These protozoan
parasites were with G. intestinalis ((P=0.002), E. histolytica/dispar (P= 0.041), Cryptosporidium
parvum (P =0.018) and Blastocystis hominis (P= 0.040).