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A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN

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Last updated: 04 Jan 2025

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Abstract

This paper describes a simple technique for staining of flatworms using lactophenol cotton blue (LPCB). The staining was tested on 2 trematode species: Heterophyes heterophyes and Mesostephanus appendiculatus, and one cestode: Diplopylidium acanthotetra, which were collected from the intestine of stray cats in Kuwait. The specimens were mounted in a small amount of the LPCB stain on a clean slide for 2-3 minutes before covering with a cover slip. The technique rapidly and clearly differentiated the internal structures of the helminthes. Its speed and simplicity are advantages over other staining methods. It is easily used in wide-scale surveys where a large number of platyhelminths have to be identified and it is suitable for field studies.

DOI

10.21608/jesp.2013.94819

Keywords

LPCB stain, flatworms, Cats, Kuwait

Authors

First Name

ADAWIA

Last Name

HENEDI

MiddleName

A.M.

Affiliation

Veterinary Laboratories, Public Authority of Agriculture Affairs and Fish Resources, P. O. Box 21422, Safat 13075, Kuwait.

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City

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Orcid

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First Name

OSAMA

Last Name

EL-AZAZY

MiddleName

M.E.

Affiliation

Veterinary Laboratories, Public Authority of Agriculture Affairs and Fish Resources, P. O. Box 21422, Safat 13075, Kuwait.

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Volume

43

Article Issue

2

Related Issue

14305

Issue Date

2013-08-01

Receive Date

2020-06-10

Publish Date

2013-08-01

Page Start

419

Page End

423

Print ISSN

1110-0583

Online ISSN

2090-2549

Link

https://jesp.journals.ekb.eg/article_94819.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=94819

Order

13

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN

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Article

Created At

22 Jan 2023