This study evaluated and revealed the consumption hazards of cattle liver infected with Fasciola spp. and revealed its effects on the serum estimation of liver enzyme (ALT) on experimental rats. A total of 42 Wister albino rats were classified into 7 groups. Four groups were fed on raw and cooked cattle liver with various intensity of Fasciola spp. infection. Two groups were fed on raw and cooked normal cattle liver (positive control), and one group served as negative control. Histopathology of the rats' liver revealed hydropic degeneration, congestion with dilatation of the central vein and sinusoids and focal areas of necrosis. The intestine samples showed degenerative changes and necrobiosis of the villar epithelium with inflammatory cell infiltration. Moreover, a slight increase was noticed in the liver enzyme ALT which is known to be an important marker of liver destruction.