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NATURAL INFECTION OF BABOONS BY ENTAMOEBA HISTOLYTICA ELICITS ANTI- GAL-LECTIN HEAVY SUBUNIT IGA AND IGG ANTIBODIES WITH SHARED EPITOPE SPECIFICITY TO THAT OF HUMANS

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Last updated: 25 Dec 2024

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Abstract

Non-human primates, such as baboons (Papio hamadryas anubis), are natural hosts for Entamoeba species; infections can be asymptomatic or result in invasive lethal disease. It was sought to determine whether following natural infection by
Entamoeba. histolytica, baboon anti-amebic antibodies recognized native Gallectin, a recombinant portion of the lectin heavy subunit (designated LC3) and specific heavy subunit epitopes; we compared the specificity of anti-amebic antibodies from baboons to that of humans following asymptomatic E. histolytica infection or cure of amebic liver abscess (ALA). Female baboons (n=54), aged one to three years of age and living in captivity were screened for infection by real time PCR. E. histolytica infection was found in 37 baboons and was associated with serum anti-LC3 IgG (73%) and anti-LC3 IgA (46%) or intestinal anti-Gal-Lectin IgA antibody responses (49%), p<0.021 for each compared to that observed with baboons having an E. dispar infection (n=10) or uninfected baboons (n=7). The ELISA OD reading for anti-LC3 or anti-lectin antibodies correlated strongly with the presence of a PCR CT value indicative of E. histolytica infection. In humans with asymptomatic E. histolytica infection or those recently cured of ALA, 63% and 57% had serum anti- LC3 IgA and 65% and 57% had serum anti-LC3 IgG antibodies respectively. Epitope- specific synthetic peptides were used as capture antigens in ELISA; for baboons that possessed anti-LC3 and anti-lectin antibodies, 74% had anti-peptide IgG or IgA antibodies, compared to 86% of asymptomatic humans and 92% of ALA subjects(P>0.05).

DOI

10.21608/jesp.2013.93319

Keywords

Entamoeba histolytica, baboons Papio, Gal-lectin, Natural infection, mucosal immunity

Authors

First Name

MOHAMED

Last Name

ABD-ALLA

MiddleName

D.

Affiliation

Department of Medicine, Medical College of Wisconsin, Egypt.

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First Name

ROMAN

Last Name

WOLF

MiddleName

F.

Affiliation

Department of Tropical Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.+, Comparative Medicine, University of Oklahoma Health Sciences Center,

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Orcid

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First Name

GARY

Last Name

WHITE

MiddleName

L.

Affiliation

Department of Tropical Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.+, Comparative Medicine, University of Oklahoma Health Sciences Center,

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First Name

STANLEY

Last Name

KOSANKE

MiddleName

D.

Affiliation

Department of Tropical Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.+, Comparative Medicine, University of Oklahoma Health Sciences Center,

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First Name

DAVID

Last Name

CAREY

MiddleName

W.

Affiliation

Department of Tropical Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.+, Comparative Medicine, University of Oklahoma Health Sciences Center,

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First Name

JACO

Last Name

VERWEIJ

MiddleName

J.

Affiliation

Department of Parasitology/Department of Medical Microbiology, Leiden University Medical Center,

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First Name

YASSER

Last Name

EL-DESSOUKY

MiddleName

M. M.

Affiliation

Department of Medicine, Medical College of Wisconsin

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City

-

Orcid

-

First Name

MIE-JIE

Last Name

ZHANG

MiddleName

-

Affiliation

Department of Population Health Biostatistics, Medical College of Wisconsin

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-

City

-

Orcid

-

First Name

JONATHAN

Last Name

RAVDIN

MiddleName

I.

Affiliation

Department of Medicine, Medical College of Wisconsin

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Volume

43

Article Issue

3

Related Issue

14073

Issue Date

2013-12-01

Receive Date

2020-06-03

Publish Date

2013-12-01

Page Start

723

Page End

735

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 43_Issue 3_Pages 723-735.pdf

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Link

https://jesp.journals.ekb.eg/article_93319.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=93319

Order

17

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Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

NATURAL INFECTION OF BABOONS BY ENTAMOEBA HISTOLYTICA ELICITS ANTI- GAL-LECTIN HEAVY SUBUNIT IGA AND IGG ANTIBODIES WITH SHARED EPITOPE SPECIFICITY TO THAT OF HUMANS

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Article

Created At

22 Jan 2023