90752

THE USE OF MICROENCAPSULATED HEPATOCYTES TRANSPLANTATION REDUCES MORTALITY AND LIVER ALTERATIONS IN SCHISTOSOMA MANSONI INFECTED HAMSTERS

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Last updated: 04 Jan 2025

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Abstract

Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotropic liver transplantation. The goal of this work was to study the adequacy of intrasplenic hepatocyte transplantation (HCTx) in fresh and microencapsulated forms, in a hamster model of liver fibrosis by Schistosoma mansoni infected hamsters were divided into 6 groups; untreated for 11 weeks (GI) and for 15 weeks (GII), treated with praziquantel (PZQ) 7 weeks PI, and killed 4 weeks (GIII) and 8 weeks (GIV) post-treatment. Treated with PZQ 7 weeks PI, and then treated orally with immunosuppressive drug "cyclosporine (4 weeks post PZQ treatment), 24 hr. before interasplenic injection with fresh hepatocytes (V). Treated with PZQ 7 weeks PI, and then injected interasplenically (4 weeks post-treatment) with microencapsulated hepatocytes (GVI). GI & GIII were killed 11 weeks PI for assessment the anti-schistosomal efficacy of PZQ. The other four groups were killed 15 weeks PI for investigation of liver and spleen histology, serum liver enzymes and hepatic oxidative markers before and after HCTx. Freshly isolated hepatocytes with a mean viability 92.971.2% were used for microencapsulation and transplantation. Histological study showed the presence of transplanted hepatocytes in spleen of recipient. PZQ accelerated healing of hepatic granulomatous lesions as evidenced parasitologically by the increase in the percentage of dead eggs and histologically showing more granuloma circumscription with more ova degeneration and less inflammatory cells. The 25-day survival rates in GII, GIV, GV& GVI were 5/15 (33.3%), 8/15 (53.3%), 10/15 (66.7%) and 9/15 (60%) respectively. In addition, there were significantly better outcomes in serum biochemical indexes such as ALT, AST, -GT, ALP, and hepatic SOD and MDA in the fresh and microencapsulated
groups than in PZQ-treated group, without great differences between the microencapsulated and the fresh transplanted groups. Liver pathological staining supported these findings.

DOI

10.21608/jesp.2014.90752

Keywords

HCTx, Schistosoma mansoni, PZQ, Microencapsulation, liver enzymes, Oxidative Stress, Lipid peroxidation

Authors

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SOAD

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SHERIF

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A.

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Department of Biochemistry, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.

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First Name

MONA

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MOHARIB

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N.

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Department of Biochemistry, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.

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First Name

NAGLAA

Last Name

EL-LAKKANY

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M.

Affiliation

Department of Pharmacology, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.

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First Name

OLFAT

Last Name

HAMMAM

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A.

Affiliation

Department of Pathology, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.

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First Name

FATMA

Last Name

SALMAN

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H.

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Department of Biochemistry, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.

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First Name

MOHAMED

Last Name

EL-NAGGAR

MiddleName

M.

Affiliation

Department of Biochemistry, Faculty of Science, Mansoura University, Egypt.

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Volume

44

Article Issue

1

Related Issue

13633

Issue Date

2014-04-01

Receive Date

2020-05-19

Publish Date

2014-04-01

Page Start

229

Page End

242

Print ISSN

1110-0583

Online ISSN

2090-2549

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https://jesp.journals.ekb.eg/article_90752.html

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https://jesp.journals.ekb.eg/service?article_code=90752

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24

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Original Article

Type Code

1,127

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Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

THE USE OF MICROENCAPSULATED HEPATOCYTES TRANSPLANTATION REDUCES MORTALITY AND LIVER ALTERATIONS IN SCHISTOSOMA MANSONI INFECTED HAMSTERS

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Article

Created At

22 Jan 2023