This study determined the prevalence of Helicobacter pylori infection in patients with different gastrointestinal symptoms. Two hundred and six patients were collected from outpatient clinic of medical department from March to June 2014. The age was ranged between 15 years old up to 60 years old. 76 males with mean age (33.2+13.5) and 130 females with
mean age (32.8+14.9). All patients were submitted to full clinical examination and stool examination was performed to detect helicobacter pylori antigen and other intestinal parasites. After getting a full history, the patient was asked specifically for history of taking nonsteroidal anti-inflammatory drugs, presence of heart burn, epigastric pain, flatulence, nausea or vomiting, passing black stool hematemesis and presence of other diseases. The results showed that 69.4% of the patients were positive for helicobacter pylori antigen (143/206). The prevalence among males and females was the same (69.7%-69.2%). The prevalence among different age groups was not significant but; some-how high among age
group of 15 up to 25 years old (70%). 72 patients out of 140 were associated with Coinfection with Entamaeba histolytica mainly or Giardia lamblia (51.4%). Epigastric pain and heart burn were representing about 90% of symptoms in patients with positive Helicobacter pylori antigen. Consequently, the prevalence of H. pylori infection is high in and around Tanta City in the Nile Delta (about 70%).