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88954

COMPARATIVE STUDY ON IMMUNOBLOT VERSUS PCR IN DIAGNOSIS OF SCHISTOSOMIASIS MANSONI IN EXPERIMENTAL INFECTED MICE

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Last updated: 25 Dec 2024

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Abstract

This study compared PCR and Western blot techniques in diagnosis of schistosomiasis mansoni. Forty Swiss albino mice were used, thirty two mice were infected with cercariae of S. mansoni and eight mice were kept uninfected which were used as a control. Blood was obtained from four infected mice weekly beginning from the 1st week to the 8th week post infection. The study found that PCR was positive from the first week post infection, while Western blot technique was positive from the second week post infection. Thus, PCR diagnosed schistosomiasis mansoni earlier than Western blot technique, but both were able to diagnose.

DOI

10.21608/jesp.2016.88954

Keywords

Egypt, Schistosoma mansoni, PCR, Western blot technique

Authors

First Name

MOUSA

Last Name

ISMAIL

MiddleName

A.M.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.

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Orcid

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First Name

WAHED

Last Name

MOUSA

MiddleName

MOHAMMED ALI

Affiliation

Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Egypt.

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Orcid

-

First Name

ENAS

Last Name

ABU-SAREA

MiddleName

YAHIA

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Beni-Suef University, Egypt.

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Orcid

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First Name

MAHA

Last Name

BASYOUNI

MiddleName

M. A.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.

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-

City

-

Orcid

-

First Name

SAMAH

Last Name

MOHAMMED

MiddleName

SAYED

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Beni-Suef University, Egypt.

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Volume

46

Article Issue

1

Related Issue

13375

Issue Date

2016-04-01

Receive Date

2020-05-13

Publish Date

2016-04-01

Page Start

101

Page End

108

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 46_Issue 1_Pages 101-108.pdf

PDF . 3.4MB

Link

https://jesp.journals.ekb.eg/article_88954.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=88954

Order

11

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

COMPARATIVE STUDY ON IMMUNOBLOT VERSUS PCR IN DIAGNOSIS OF SCHISTOSOMIASIS MANSONI IN EXPERIMENTAL INFECTED MICE

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Article

Created At

22 Jan 2023