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88809

ALTERNATIVE STAINING OF FASCIOLA GIGANTICA USING NATURAL HERBAL DYES

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Last updated: 04 Jan 2025

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Abstract

The Aqueous extracts of red beet, China rose, henna and acid carmine stains were used to stain the adult worms of F. gigantca. The stained flukes showed varying degrees of pigmentation with well-defined external and internal structure. Oral and ventral sucker, muscular pharynx, oesophagus, intestinal ceca and cirrus sac took rose to light cherry color in aqueous extract of china rose, orange to brown color in aqueous extract of sugar beet, beige to pale brown color in aqueous extract of henna while they took deep red color in carmine stain with best quality achieved by china rose aqueous extract. The ovary, testes and longitudinal excretory canal/pore took rose to cherry color in aqueous extract of China rose, pale brown color in aqueous extracts of both sugar beet and henna while they acquired red to pink color in acid carmine stain. Aqueous extracts of sugar beet exhibited little better quality in revealing characteristics of vitelline ducts and
vitelline glands compared to the aqueous extract of China rose, henna and carmine stain. Uterus stained brown in both sugar beet aqueous extracts and carmine stain while it took yellow color when stained with China rose and henna. Eggs inside uterus took light yellow color in all tested aqueous extracts. Ootype/mehli gland and yolk reservoir of F. gigantica took brown color in aqueous extracts of red beet, China rose and henna, red to pink color in carmine stain. Spines
covering the cuticle appeared rose color in aqueous extract of china rose, pale brown color in aqueous extract of sugar beet extract and henna, and pale red color in carmine stain.

DOI

10.21608/jesp.2020.88809

Keywords

Fasciola gigantica, Natural herbal dyes

Authors

First Name

MARWA

Last Name

ALY

MiddleName

E.

Affiliation

Animal Health Research Institute, Faculty of Medicine, Assuit University, Egypt.

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First Name

AHMED

Last Name

DYAB

MiddleName

K.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.

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City

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Orcid

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First Name

DOAA

Last Name

YONES

MiddleName

A.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.

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City

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Orcid

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First Name

YASSER

Last Name

MOHAMED

MiddleName

M.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.

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City

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Orcid

-

First Name

SOAAD

Last Name

BAYOUMI

MiddleName

A.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assuit University, Egypt

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City

-

Orcid

-

First Name

NAHED

Last Name

ELOSSILY

MiddleName

A.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.

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City

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Orcid

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Volume

50

Article Issue

1

Related Issue

13315

Issue Date

2020-04-01

Receive Date

2020-05-12

Publish Date

2020-04-01

Page Start

127

Page End

134

Print ISSN

1110-0583

Online ISSN

2090-2549

Link

https://jesp.journals.ekb.eg/article_88809.html

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https://jesp.journals.ekb.eg/service?article_code=88809

Order

17

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

ALTERNATIVE STAINING OF FASCIOLA GIGANTICA USING NATURAL HERBAL DYES

Details

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Article

Created At

22 Jan 2023