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88270

COMPARISON BETWEEN KATO-KATZ THICK SMEAR AND SEDIMENTATION TECHNIQUES IN DIAGNOSIS OF FAECAL-ORALLY TRANSMITTED HELMINTHES AND OTHER GEOHELMINTHES

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Last updated: 25 Dec 2024

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Abstract

Kato-Katz technique is widely used for the diagnosis of Fecal-orally transmitted helminthic infections. It is relatively simple and inexpensive. However, a single slide prepared from a single stool specimen has low sensitivity, particularly in light infections. Therefore, there is a great need for concentration techniques. This study detected an accurate and affordable method for diagnosis of fecal-orally transmitted helminthes and other geohelminthes. The study was carried out on 217 stool samples of different sex and age groups. Stool samples were collected from different urban and rural areas in Sharkia Governorate. Stool samples were examined macroscopically and analyzed by different parasitological techniques: direct wet mount (DWM), Kato-Katz thick smear, spontaneous sedimentation in tube technique (SSTT) and formol ether sedimentation technique (FEC). The results showed that formol ether sedimentation method detected 59 positive samples
followed by spontaneous sedimentation in tube technique (48/59 positive samples). FEC showed significant difference when compared to direct wet mount and Kato-Katz thick smear. SSTT also showed significant difference when compared to DWM (P <0.05).The overall prevalence of intestinal helminthes was (29.6%) among studied samples in Sharkia
Governorate. The commonest helminthic infection was H. nana (12%) followed by E. vemicularis(10%) then A. lumbricoides (3.7%).

DOI

10.21608/jesp.2016.88270

Keywords

Geohelminthes, Kato-Katz thick smear, direct wet mount methods, Formol ether concentration method

Authors

First Name

ALAAELDIN

Last Name

ELGENDY

MiddleName

M.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt

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First Name

FATEN

Last Name

MOHAMMED

MiddleName

A.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt

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First Name

ASHRAF

Last Name

METWALLY

MiddleName

S.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt

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City

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First Name

RANIA

Last Name

HAMZA

MiddleName

S.

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt

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First Name

SAMAR

Last Name

KAMEL

MiddleName

-

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt

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Volume

46

Article Issue

3

Related Issue

11654

Issue Date

2016-12-01

Receive Date

2020-05-10

Publish Date

2016-12-01

Page Start

647

Page End

654

Print ISSN

1110-0583

Online ISSN

2090-2549

Link

https://jesp.journals.ekb.eg/article_88270.html

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https://jesp.journals.ekb.eg/service?article_code=88270

Order

21

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

COMPARISON BETWEEN KATO-KATZ THICK SMEAR AND SEDIMENTATION TECHNIQUES IN DIAGNOSIS OF FAECAL-ORALLY TRANSMITTED HELMINTHES AND OTHER GEOHELMINTHES

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Article

Created At

22 Jan 2023