Parasitic infection is still a serious public health problem in the world, especially in developing countries including Egypt. It represents a major cause of morbidity and mortality in childhood and among high-risk groups in most parts of the world. This study detected the prevalence of parasitic infection among school children in El-Wadi El-Gadded (the New Valley Governorate). A total of randomly chosen 1615 students aged from 6-16 years, (771 males & 844 female) from 12 primary schools and 12 preparatory schools related to four centers (El Dakhala, El Farfra, Paris and Plat) from the New Valley Governorate. Each child was subject to: A questionnaire sheet, Urine examination through sedimentation methods, Stool examination using: Direct smear, Simple sedimentation method and Modified Ziehl-Neelsen Stain, Blood samples were collected randomly from 450 children and examined for Seropositivity of toxoplasmosis using (On-SiteToxoIgG/IgM Rapid Test- Cassette) and examination of hair & clothes for ectoparasites (lice). The overall prevalence of parasites was (39.1%) among primary and preparatory school children. The helminthes were E. vermicularis (15.2% & 17.1%); A. lumbricoides (1.3% & 1.9%) and then H. nana (0.9% & 0.6%) and the protozoa were E. histolytica (14.1% & 13.2%), Giardia lamblia (3.8% & 3.9%), and then Cryptosporidium parvum (0.09%) and seropositivity of toxoplasmosis was in (3.0% & 2.7%) among primary and preparatory school children respectively. Mixed infection was in (0.4%) among primary school children. Head lice infestation was more prevalent among primary school children than preparatory school ones with a ratio (3.5% &0.2%) respectively, was nil among males.