This study evaluated the association between expression of Twist2 monogenic, with pathological features and clinical outcomes of squamous cell carcinoma of the urinary bladder (SCCUB) following radical cystectomy (RC). Immunohistochemical staining Twist2 was performed on tissue archival samples comprising normal urothelium from ten controls, cystectomy specimens from 87 patients with T2 N0 M0 and T3-4a N0 M0 of bladder squamous cell carcinoma
(SCC).all patients with T2-4 SCC undergone radical cystectomy and urinary diversion, follow up was for 5-10 years to assess overall survival (OS) and disease free survival (DFS). Specimens from 10 muscle-invasive urothelial carcinoma patients were reassessed for Twist2 expression to represent over- expression of Twist2. All ten controls had normal status of biomarkers which was negative. Negative or, low, and medium expression of Twist2 was noted in SCC of the urinary bladder of local pathological status with no lymph node metastasis or distant metastasis, clinicopathologic characteristics of the cohort were pT2N0 M0 (n=659 and pT3-4a N0 M0 (n= 22). Twist2 negative expression was in 36 case, weak expression (n=33), medium expression (n=16), there were no strong expression. Twist2 low-regulation with combination of tomor stage in SCC of the urinary bladder werel independently associated with overall survival and free disease survival, 7.7 and 5 years survival in pT2 N0 M0(n=65) were 89% and 13% respectively, 7.7 and 5 years survival in pT3-4a N0 M0(n=22) were 72.7% and 22.7% respectively.