Protozoan parasite Toxoplasma gondii is considered as one of the most critical risk factor for recurrent abortion in pregnant females and resulted in multi congenital malformation in fetus world-wide. The present study was carried out on 693 pregnant females from Alexandria, Beheira, Gharbia, Menoufia, Qalyoubia and Fayoum provinces. The study determined the prevalence of toxoplasmosis in pregnant females during pregnancy trimesters and shed the lights on
the main risk factors and possible contamination routes. Detection for the presence of Toxoplasma IgG antibodies were done by enzyme linked fluorescence assay (ELFA). It was found that the overall seroprevalence of T. gondii was 30.16%. The seroprevalence increased with age. Significant relations were observed between Toxoplasma IgG antibodies and abortion history, maternity trimester and consumption of under-cooked meat. No significant differences were reported
due to parity, occupation, abortion trimester, contact with cats and/or other animals and exposure to soil.