Needle stick injuries are a common event in the healthcare environment. When drawing blood, administering an intramuscular or intravenous drug, or performing other procedures involving sharps, needle can slip and injure the healthcare worker. This allows for transmission of pathogens. Injuries also occur during needle recapping or failure to place used needles in the sharps containers. Also, lack of access to or failure to use appropriate personal protective equipment can cause needle stick injuries. Night shifts also put practitioners at risk for needle stick injuries. The study assessed the effect of an intervention program for nursing staff knowledge, performance and attitude related to blood protozoa acquired by needle stick injury. Design an interventional study (pre-post study) was used for 90 nursing staff, ten of them pilot study were excluded. The study tools composed of educational needs assessment tool, knowledge questionnaire sheet (pre/post-test), observation check list and attitude Results showed significant improvements in nursing staff knowledge, performance and attitude.