Toxoplasma gondii is a critical zoonotic protozoan infection associated with comorbidity on pregnancy and fetus. This study evaluated over a year the sero-prevalence of T. gondii among multiparous pregnant women undergoing antenatal care at Beni-Suef University's Hospital, and estimated the risks for infection. All patients were subjected to immunological detection of both IgG & IgM anti-toxoplasmosis antibodies using ELISA. Out of 300 females, anti-T.gondii antibodies were detected in 20% (n=60) of cases. Of 60 seropositive cases, IgG+ve, IgM+ve and both IgG+ve and IgM+ve were detected in 56.6% (n=34), 23.3% (n=14) and 20% (n=12) respectively. Positive cases were common in age groups 30-40 (45%) and 20-30 (43.3%). 50% of positive patients suffered from first trimester abortion (p=0.005). History of the previous obstetric outcome was a predictive factor (p =0.002) for infection. None of other variables were risks.