Cystic echinococcosis (CE) is one of the commonest zoonotic parasitosis of worldwide distrib-ution. This study induced a protective immunity against secondary hydatidosis by using conven-tional vaccination approaches and evaluated the accompanied humeral immune response. For antigen preparation Hydatid cysts (HC) were collected from camels slaughtered at Cairo abattoir, during 2015.Three groups of male rabbits were immunized subcutaneously with crude Hydatid cyst fluid (CHCF), partially purified Hydatid cyst fluid (ppHCF) and protoscolices (PSC) antigens. After two boosters, rabbits were challenged intra peritoneally with 2000 viable protoscolices, and the humeral immune response
was analyzed using ELISA. Percent of protection against HC was 93.0 %, 88.4 % and 86.0 % in immunized rabbits with ppHCF, PSC and CHCF antigens respectively. Antibody level in immunized group with ppHCF antigen on day 28 was higher than before immunization and was higher than that in CHCF and PSC antigen groups. The results of this article indicate that ppHCF antigen can be used as a candidate for vaccine production.