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EVALUATION OF FECONOMICS AND MINI-FLOTAC AS RECENT STOOL PREPARATION TECHNIQUES FOR DIAGNOSIS OF INTESTINAL PARASITES

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Last updated: 04 Jan 2025

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Abstract

Till now there is no gold standard method for diagnosis of intestinal parasitic infections. The present work evaluated the performance of recently developed methods; mini-FLOTAC and Feconomics and to compare them with the old traditional methods. A total of 136 stool samples (collected from diarrheic patients) were analyzed by the newly developed techniques; Feconomics and mini-FLOTAC with three flotation solutions (FS2, FS6 & FS7) and traditional methods; direct smear and formol ether concentration method (FECM). The total number of positive samples detected by any of the methods was taken as diagnostic “gold" standard for each parasite species (combined results). Sensitivity, negative predictive value and kappa index of agreement were calculated for each method compared to combined results in detection of all parasites. Overall, 93.4 % of the cases were found to be infected with parasites. The most prevalent parasite detected was Cryptosporidium (25.7 %) while the least detected parasitic infection was Trichostrongylus colubriformis (8.1%). The most sensitive method for protozoa detection was Feconomics followed by FECM and mini-FLOTAC FS7 (97%, 55% & 47% respectively). Also, Feconomics method was the most sensitive for detection of helminths followed by mini-FLOTAC FS7 and mini-FLOTAC FS2 (94 %, 79 % & 72 %). Feconomics showed good agreement in detection of all parasites while it showed perfect agreement for individual parasite with superiority to all other methods. Mini-FLOTAC showed better performance in detection of helminths compared to protozoa. Feconomics method is a promising technique for detection of all intestinal parasites. It is simple, highly sensitive, time saving and does not require centrifugation. More studies are still needed to clarify the effectiveness of both methods.

DOI

10.21608/jesp.2018.77477

Keywords

Parasites, Diagnosis, Feconomics, Mini-FLOTAC

Authors

First Name

MOHAMED

Last Name

ABDEL-GAFFAR

MiddleName

M.

Affiliation

Department of Parasitology, Faculty of Medicine, Menoufia University, Egypt.

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Orcid

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First Name

GEHAN

Last Name

SADEK

MiddleName

S.

Affiliation

Department of Parasitology, Faculty of Medicine, Menoufia University, Egypt.

Email

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City

-

Orcid

-

First Name

SALWA

Last Name

OSHIBA

MiddleName

F.

Affiliation

Department of Parasitology, Faculty of Medicine, Menoufia University, Egypt.

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-

City

-

Orcid

-

First Name

EMAN

Last Name

ESMAIL

MiddleName

S.

Affiliation

Department of Parasitology, Faculty of Medicine, Menoufia University, Egypt.

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-

City

-

Orcid

-

Volume

48

Article Issue

1

Related Issue

11519

Issue Date

2018-04-01

Receive Date

2020-03-16

Publish Date

2018-04-01

Page Start

139

Page End

146

Print ISSN

1110-0583

Online ISSN

2090-2549

Link

https://jesp.journals.ekb.eg/article_77477.html

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https://jesp.journals.ekb.eg/service?article_code=77477

Order

16

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

EVALUATION OF FECONOMICS AND MINI-FLOTAC AS RECENT STOOL PREPARATION TECHNIQUES FOR DIAGNOSIS OF INTESTINAL PARASITES

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Article

Created At

22 Jan 2023