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77476

THE PROTECTIVE EFFECT OF INSULIN AGAINST ISOFLURANE AND SEVOFLURANE INDUCED HEPATOCELLULAR APOPTOSIS

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Last updated: 04 Jan 2025

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Abstract

Several studies deducted that inhalational anesthetics induce apoptosis in human cells. Insulin is believed to have an antiapoptotic action so it is widely used as cardioprotective agent against ischemic reperfusion injuries. This study compared the apoptotic effect of inhalational anesthetics and figuring out the antiapoptotic effect of insulin against perioperative induced hepatocellular apoptosis using immune histochemical study of liver biopsy. Eighty (ASA I) patients scheduled for laparoscopic cholecystectomy were randomly allocated into 4 groups (20 patients each). Two groups were anesthetized using isoflurane and the other two were anesthetized using sevoflurane. The control groups (IC, SC) received normal saline and the insulin groups (II, SI) received glucose, insulin and potassium (GIK) infusion. Infusions were given 2 hours before induction of anesthesia. Liver biopsy was taken before the umbilical port closure. In liver biopsy Caspase 3, 7, 9 and Akt activity were evaluated. Liver function tests were estimated before infusion and one day after surgery. Serum K and blood glucose levels were closely monitored all through the study. The results showed that in the isoflurane groups, the percentage of caspase 3 and 7 positive cases decreased while Akt positive cases increased significantly in II compared to IC respectively (p < 0.05). In the sevoflurane groups, the percentage of caspase 3 positive cases decreased
significantly in SI compared to SC group (p < 0.05).

DOI

10.21608/jesp.2018.77476

Keywords

protective, perioperative, hepatocellular, apoptosis, Insulin

Authors

First Name

KHALDA

Last Name

RADWAN

MiddleName

G.

Affiliation

Department of Anesthesia &amp; SICU, Faculty of medicine Cairo University, Giza, Egypt.+, Theodor Bilharz Research Institute, Imbaba B.O. Box 30, Giza12411, Egypt.

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First Name

MOSHIRA

Last Name

SAYED

MiddleName

-

Affiliation

Department of Anesthesia &amp; SICU, Faculty of medicine Cairo University, Giza, Egypt.+, Theodor Bilharz Research Institute, Imbaba B.O. Box 30, Giza12411, Egypt.

Email

-

City

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Orcid

-

First Name

WAEL

Last Name

EL-SIORY

MiddleName

A.

Affiliation

Department of Anesthesia & SICU, Faculty of medicine Cairo University, Giza, Egypt.

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-

City

-

Orcid

-

First Name

MOHAMED

Last Name

MAHER

MiddleName

A.

Affiliation

Department of Anesthesia &amp; SICU, Faculty of medicine Cairo University, Giza, Egypt.+, Theodor Bilharz Research Institute, Imbaba B.O. Box 30, Giza12411, Egypt.

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Orcid

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First Name

MOHAMED

Last Name

HUSSEIN

MiddleName

M.

Affiliation

Department of Anesthesia &amp; SICU, Faculty of medicine Cairo University, Giza, Egypt.+, Theodor Bilharz Research Institute, Imbaba B.O. Box 30, Giza12411, Egypt.

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-

City

-

Orcid

-

First Name

OLFAT

Last Name

HAMMAM

MiddleName

A.

Affiliation

Department of Pathology, Theodor Bilharz Research Institute, Imbaba B.O. Box 30, Giza12411, Egypt.

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-

Orcid

-

First Name

MONA

Last Name

HASSAN

MiddleName

-

Affiliation

Department of Clinical Chemistry, Theodor Bilharz Research Institute, Imbaba B.O. Box 30, Giza12411, Egypt.

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-

City

-

Orcid

-

First Name

AHMED

Last Name

HELMY

MiddleName

H.

Affiliation

Department of General surgery, Theodor Bilharz Research Institute, Imbaba B.O. Box 30, Giza12411, Egypt.

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-

City

-

Orcid

-

First Name

HODA

Last Name

OKASHA

MiddleName

H.

Affiliation

Department of Anesthesia & SICU, Faculty of medicine Cairo University, Giza, Egypt.

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-

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Volume

48

Article Issue

1

Related Issue

11519

Issue Date

2018-04-01

Receive Date

2020-03-16

Publish Date

2018-04-01

Page Start

129

Page End

137

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 48_Issue 1_Pages 129-137.pdf

PDF . 826.9kB

Link

https://jesp.journals.ekb.eg/article_77476.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=77476

Order

15

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

THE PROTECTIVE EFFECT OF INSULIN AGAINST ISOFLURANE AND SEVOFLURANE INDUCED HEPATOCELLULAR APOPTOSIS

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Article

Created At

22 Jan 2023