One hundred female patients (age 18-50yrs) were enrolled in this study. Females were nonmenstruating and non-pregnant with no intercourse or douching for the last 2 days, no use of antiprotozoa, antibiotics and steroids for the past 2 weeks and complaining mainly of vaginal discharge, itching or both. Vaginal swabs were obtained from all patients and examined for Trichomonas vaginalis by direct wet mount, Giemsa staining, modified Diamond's culture medium and Latex agglutination test (Kalon). Results: Out of 100 patients, T. vaginalis was found in 8 (8%) patients by any of the used diagnostic methods. Latex agglutination test detected 8 positive cases, 6 of which were also positive by Diamond's culture, 3 cases were detected by Giemsa staining and only one case was detected by wet mount. The wet mount, Giemsa staining and Kalon latex test had sensitivities of 16.7%, 50% and 100% respectively and accuracy rates 95.2%, 97.1% and98% respectively as compared to the modified Diamond's culture as the gold standard. Meanwhile all of them had specificities 100% except for latex agglutination test, it was 97.9%.There was no significant association between potentially probable risk factors and confirmed trichomoniasis detected by Diamond's culture (the gold standard test) except in patients being from urban areas and complaining of either dysuria or dyspareunia or both.