The present study focused on dipterous flies, distribution and their densities in nine centers of Menoufia Governorate, Egypt (Quesna, Shebeen El-Kom, Berkat El-Sabe, El-Bagour, Al-Shohada, Tala, Menof, Al-Sadat and Ashmon). The result indicated that 30 species belonging to 22 genera and 11 families were trapped from Menoufyia Governorate in spring 2015. Musca domestica, Coproica vegans, Cop. Ferruginata, Sepsis thoracica, S. lateralis and S.
fissa were the most abundant species in the Governorate. The results showed that there was no statistically significant according to the total number of flies species between centers (F=0.794 & P=0.686). Data also indicated that there was statistically significant between species according to the total number of flies in Quesna center (F=2.576; P=0.040), but there was no statistically significant between species according to the total number of flies to other centers, Shebeen El-Kom (F=1.368; P=0.282), Berkat El-Sab (F=2.00; P=0.95), El-Bagour (F=0.771; P=0.95), Al-Shohada (F=0.900; P=0.564), Tala (F=1.926; P=0.110), Menof (F=1.640; P=0.170), Al-Sadat (F=1.537; P=0.216), Ashmon (F=0.611; P=0.759). Shebeen El-Kom Center showed the highest prevalence of the species of the most families due to suitable
breeding sites. Ashmon showed the lowest prevalence of the most species.