Lung and colorectal cancer represent a major health problem worldwide. This study evaluated the cytotoxic effect, anti-cancer properties of beetles derived chitosan. The study conducted using lung (A549) and colorectal (HCT-116) cancer cell lines to identify the anti-cancer effect. Cytotoxicity was evaluated by describing and measuring recoding morphological changes. The viability and related IC50 were cell type and concentration dependent. Also, related cell apoptosis was monitored using PI stain where early and late apoptosis of treated A549 cells was significantly elevated than in case of HCT-116 cell line (P<0.05) than control. In the meantime, the necrotic % of treated cells didn't perform any changes between the two cell lines but significantly elevated than that of cell control (P<0.05). Apoptotic profile was examined and showed up regulation of both proapoptotic genes (P53 and Bax) accompanied with down regulation of BCL-2 in both cell lines.