Beta
ManageSearchChatDiscover
76901

MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTA

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Trypanosoma evansi is the most widespread of the pathogenic salivarian trypanosomes and cause a serious disease called (surra) that is affect the domestic animals such camels and horses in Tropical and subtropical countries and often leads to reduced productivity and economic losses. Therefore, the objectives of the present study were to determine the prevalence rates of trypanosomiasis using polymerase chain reaction (PCR) among camels from five different regions of Saudi Arabia and to sequence and characterized the T. evansi from these animals. In the current study, 832 camel blood samples collected from five different regions of Saudi Arabia for detecting T. evansi. A generic ITS1-PCR and RoTat 1.2 VSG gene were applied in this study to analyze camels' blood samples. Molecular analysis was performed using ITS1-PCR
which showed that the highest prevalence of trypanosomes was observed in Al-Qaseem province (50.1%) followed Riyadh province (49%), whereas in Hail and the Northern Borders, there were fewer infections with trypanosomes (28.4% &17.6%), respectively. PCR amplification was carried out targeting RoTat 1.2 VSG gene on TS1-positive samples and some of them were negative for RoTat1.2. The test negative in RoTat 1.2 PCR but ITS1 PCR positive could suggest T. evansi type B. Presence of T. evansi type B is interest to the international community, as this has a message to redesign the existing molecular and serological diagnostic markers. However, to our knowledge this the first study demonstrating T. evansi type B out of Africa.

DOI

10.12816/0050431

Keywords

Trypanosoma evansi, camels, ITS1 rDNA, RoTat 1.2, PCR, Saudi Arabia

Authors

First Name

ABDULLAH

Last Name

ALANAZI

MiddleName

D.

Affiliation

Department of Biological Science, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911.

Email

-

City

-

Orcid

-

First Name

R.

Last Name

PUSCHENDORF

MiddleName

-

Affiliation

School of Biological Sciences, Plymouth University, Drake Circus, Plymouth, PL4 8AA, UK.

Email

-

City

-

Orcid

-

First Name

MOHAMED

Last Name

ALYOUSIF

MiddleName

S.

Affiliation

Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451.

Email

-

City

-

Orcid

-

First Name

MOHAMED

Last Name

AL-KHALIFA

MiddleName

S.

Affiliation

Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451.

Email

-

City

-

Orcid

-

First Name

SAMIR

Last Name

ALHARBI

MiddleName

A.

Affiliation

Department of Biological Science, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911.

Email

-

City

-

Orcid

-

First Name

ZAFER

Last Name

AL SHEHRI

MiddleName

S.

Affiliation

Department of Biological Science, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911.

Email

-

City

-

Orcid

-

First Name

ASHRAF

Last Name

SAID

MiddleName

E.

Affiliation

Department of Biological Science, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911.+, Department of Zoology, Faculty of Science, Damietta University, New Damietta, Damietta, Egypt.

Email

-

City

-

Orcid

-

First Name

IBRAHIM

Last Name

ALANAZI

MiddleName

O.

Affiliation

The National Centre for Genomic Technology, King Abdulaziz City for Science and technology, P.O. Box 6086, Riyadh 11442.

Email

-

City

-

Orcid

-

First Name

HAMDAN

Last Name

AL-MOHAMMED

MiddleName

I.

Affiliation

Department of Parasitology, Faculty of Medicine, King Faisal University P.O. Box 400, AlAhsa 31982, Saudi Arabia.

Email

-

City

-

Orcid

-

Volume

48

Article Issue

2

Related Issue

11512

Issue Date

2018-08-01

Receive Date

2020-03-11

Publish Date

2018-08-01

Page Start

241

Page End

250

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 48_Issue 2_Pages 241-250.pdf

PDF . 854.9kB

Link

https://jesp.journals.ekb.eg/article_76901.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=76901

Order

1

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTA

Details

Type

Article

Created At

22 Jan 2023