This studied the medical complains in group of slaughter house workers in daily contact to slaughtered buffaloes. Carcasses of 400 slaughtered buffaloes were examined for Sarcocystis muscle cyst infection. Bradyzoites extracted from the diagnosed Sarcocystis muscle cyst (SMC) were used for production of Sarcocystis bradyzoites antigens (SBAg). The antigen was fractionated. Eluted concentrated specific fractions of 35-63 Kda molecular weight (MW) after EITB were used for infection sero-diagnosis of slaughtered buffaloes and Slaughter house workers using Dot ELISA. Data revealed that infection in slaughtered buffaloes was 36.57% by macro and microscopic investigation. Anti-Sarcocystis anti-bodies (ASAb) by dot ELISA were 52.5% among buffaloes. Sensitivity was 100%, specificity was 86.66% with 93.33% validity.
Using the previous technique in examination of group of slaughter house workers revealed high association between presence of ASAb in sera of patients complained from Myositis and arthritis (66.0%) followed by those complained by Myositis (50%) only, while it was 44.0% in workers complained by arthritis only. No ASAb was recorded in 4th group complained by digestive disturbances only as well as in control healthy individuals.