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76553

HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN

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Last updated: 25 Dec 2024

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Abstract

Cryptosporidiosis is a recognized child infectious killer and the second cause of diarrheal disease and death in infants. Assessing Cryptosporidium spp. genetic diversity is a real goal to elucidate its transmission dynamics and to design preventive measures in absence of effective treatment. Cryptosporidium isolates in stool of Egyptian children were detected using Acid Fast (AF) staining, copro-nPCR/RFLP assay and real time PCR high-resolution melting (HRM) curve analysis assay. Stool samples were collected from 335 children complaining of diarrhea and other GIT symptoms, attending the outpatient clinic of Abu El Reesh hospital, Kasr Al-Ainy School of Medicine, Cairo University. Two genotypes C. hominis and C. parvum were identified in 43 isolates from Egyptian children by copro-nPCR targeting COWP gene and HRM assay. Real time PCR HRM curve analysis, a closed-tube genotyping method, targeting ITS-2 gene confirmed the results of copro-nPCR/RFLP. It is simple, rapid, has more sample throughput, analysis capacities and data storage with less carry-over contamination and cost.

DOI

10.21608/jesp.2018.76553

Authors

First Name

MARWA

Last Name

GHALLAB

MiddleName

M. I.

Affiliation

Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University.+, Department of Medical Parasitology, Faculty of Medicine, Kafr El- Sheikh University, Egypt.

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First Name

MONA

Last Name

KHATER

MiddleName

M.

Affiliation

Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.

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Orcid

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First Name

KEVIN

Last Name

TYLER

MiddleName

M.

Affiliation

Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, UK.

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Orcid

-

First Name

MAHA

Last Name

BOUZID

MiddleName

-

Affiliation

Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, UK.

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Orcid

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First Name

AZZA

Last Name

ABD ELMEGIED

MiddleName

KAMAL

Affiliation

Department of Pediatrics, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.

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Orcid

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First Name

AYMAN

Last Name

EL-BADRY

MiddleName

A.

Affiliation

Department of Medical Parasitology, Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Fasial University, Dammam, Saudi Arabia.

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Volume

48

Article Issue

3

Related Issue

11474

Issue Date

2018-12-01

Receive Date

2020-03-09

Publish Date

2018-12-01

Page Start

557

Page End

562

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 48_Issue 3_Pages 557-562.pdf

PDF . 534.6kB

Link

https://jesp.journals.ekb.eg/article_76553.html

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https://jesp.journals.ekb.eg/service?article_code=76553

Order

9

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN

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Article

Created At

22 Jan 2023