The present work investigated the larvicidal efficacy of alkaloids extracted from Lupin seeds by water (aqueous extract) and methanol (methanol extract) against mosquitoes larvae (Culex pipiens), flies larvae (Musca domestica) and fleas larvae (Xenopsylla cheopis) under laboratorycondition in Egypt. Alkaloids were analyzed by gas chromatography – mass spectrometry (GCMS). Analysis revealed the presence of lupanine (100%), 13-OH-Lupanine (16.48%) and13α- Angeloyloxylupanine (1.3%) in methanol extract of Lupinus luteus and α- isolupanine (100%) only in aqueous extract of lupinus luteus. The lethal concentration LC50 and LC90 were obtained from the established regression log concentrate-response lines after 24 hours. Data indicated that the efficacy of methanol extract of alkaloids against Culex pipiens larvae was more effective than the effect of aqueous extract of alkaloids. The values of lethal concentration of methanol extract of alkaloids were 0.79 and 1.17 mg/ml for LC50 and LC90 respectively, while the values of lethal concentration of aqueous extract of alkaloids were 5.43 and 8.50 mg/ml for LC50 & LC90 respectively. Data also indicated that the efficacy of methanol extract of alkaloids against Musca domestica larvae was effective than the effect of aqueous extract of alkaloids. The values of lethal concentration of methanol extract of alkaloids were 6.40 and 19.18 mg/ml for LC50 &LC90 respectively, while the same values of aqueous extract of alkaloids were 11.20 and 34.00 mg/ml for LC50 and LC90 respectively. In addition, the efficacy of methanol extract of alkaloids against Xenopsylla cheopis larvae was more effective than the effect of aqueous extract of alkaloids. The lethal values concentration of alkaloids methanol extract were 10.56, 19.20 and mg/ml for LC50 &LC90 respectively, but alkaloids values of aqueous extract were 23.21 and 41.69 mg/ml for LC50 & LC90 respectively.