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68145

ELECTRON MICROSCOPY OF BLASTOCYSTIS HOMINIS AND OTHER DIAGNOSTIC APPROACHES

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Last updated: 25 Dec 2024

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Abstract

This work evaluated light microscopy and culture against conventional PCR in diagnosing Blastocystis hominis in human stool samples, and were studied by scanning and transmission electron microscope. Hundred stool samples were acquired from the Parasitology laboratory of the Medical Research Institute in Alexandria. All samples were subjected to light microscopy, culture and conventional PCR to diagnose B. hominis in human stool samples; subsequently B. hominis obtained from culture media were further studied by scanning and transmission electron microscope.
The results showed B. hominis was detected in 52/100 studied stool samples using light microscope, xenic culture showed 65 positive samples, 67 samples were positive by conventional PCR. SEM revealed the outer surface of B. hominis and rosette-shaped dividing oocysts while TEM demonstrated internal structures of the organisms.

DOI

10.21608/jesp.2019.68145

Keywords

Blastocystis hominis, Diagnosis, Microscopy, culture, PCR

Authors

First Name

MONA

Last Name

ELSAYAD

MiddleName

HASSAN

Affiliation

Department of Parasitology, Medical Research Institute, Alexandria University, Egypt.

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First Name

MONA

Last Name

TOLBA

MiddleName

MOHAMED

Affiliation

Department of Parasitology, Medical Research Institute, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

HANIA

Last Name

ARGIAH

MiddleName

AHMED

Affiliation

Department of Parasitology, Medical Research Institute, Alexandria University, Egypt.

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-

City

-

Orcid

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First Name

AHMED

Last Name

GABALLAH

MiddleName

-

Affiliation

Department of Microbiology, Medical Research Institute, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

MERVAT

Last Name

OSMAN

MiddleName

MOSTAFA

Affiliation

Department of Parasitology, Medical Research Institute, Alexandria University, Egypt.

Email

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City

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Orcid

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First Name

IRENE

Last Name

MIKHAEL

MiddleName

LEWIS

Affiliation

Department of Hematology, Medical Research Institute, Alexandria University, Egypt.

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-

City

-

Orcid

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Volume

49

Article Issue

2

Related Issue

10317

Issue Date

2019-08-01

Receive Date

2020-01-14

Publish Date

2019-08-01

Page Start

373

Page End

380

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 49_Issue 2_Pages 373-380.pdf

PDF . 522.4kB

Link

https://jesp.journals.ekb.eg/article_68145.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=68145

Order

15

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

ELECTRON MICROSCOPY OF BLASTOCYSTIS HOMINIS AND OTHER DIAGNOSTIC APPROACHES

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Article

Created At

22 Jan 2023