Introduction: The stability of miniscrew implants (MIs) is governed by the clinical, biomechanical and biochemical assessments.
Purpose: to evaluate Osteopontin (OPN) and Periostin (PSN) levels in peri-miniscrew implant crevicular fluid (PMICF) at different time intervals. Subjects and methods: Eight skeletal Class III patients with maxillary deficiency were selected. Sixteen MIs (Hubit co, Korea) of 1.6 mm diameter and10 mm length were placed between upper second premolars and first molars. Additional sixteen MIs of 1.4 mm diameter and 8 mm length were inserted between lower canines and first premolars. A fixed posterior bite plate was placed and 300g force was immediately delivered by intermaxillary closed coil springs (Ortho Technology, TAD coil spring, USA). PMICF samples were obtained before loading(T1); on day one(T2), two(T3), seven(T4) and on day 30 (T5) after force application. Enzyme-linked immunosorbent assay (ELISA) kits were used.
Results: The percentage change in levels of OPN and PSN broadly showed a decrease upon loading of MIs. However, at the end of observation period, the difference between T1and T5 was statistically insignificant.
Conclusions: The OPN and PSN levels varied around MIs as a result of force application and may be used as biomarkers for assessing implant stability throughout loading periods. Immediate loading of MIs with intermaxillary closed coil springs for treatment of skeletal Class III patient did not impair implant stability.