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78681

Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3

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Last updated: 25 Dec 2024

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Abstract

 
 Objective: To investigate the time course of osteocyte apoptosis in a rat model of orthodontic tooth movement as revealed by the caspase-3 activation pathway. Materials and Methods: Fifteen male Sprague Dawley rats (6–9weeks old) were loaded with an orthodontic appliance. A spring delivering 10–12 g of force was placed between the maxillary first molar and the incisor to displace the first molar mesially. The animals were equally divided into three time points: 24 hours, 3 and 7 days of orthodontic loading. Five animals served as 0 day group. Caspase 3 immunostaining, was performed on histologic sections of the first molars. The labeling was quantified in osteocytes on the mesial and distal sides of the alveolar bone in each group. Results: Caspase 3 labeling at the mesial side significantly increased at 1, 3 and 7 days after orthodontic loading compared to the control group. No peak increase was observed. There was no significant difference in caspase 3 labeling at the distal side. Conclusions: Osteocyte apoptosis during orthodontic tooth movement could be revealed by caspase 3 immunostaining. This suggest that caspase 3 apoptotic pathway is involved in osteocyte death during orthodontic tooth movement.

DOI

10.21608/eos.2016.78681

Keywords

Osteocyte, apoptosis, Caspase 3, orthodontic tooth movement

Authors

First Name

Hassan

Last Name

Kassem

MiddleName

-

Affiliation

Department of Orthodontics, Faculty of Dentistry, Alexandria University.

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City

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Orcid

-

First Name

Iman

Last Name

Talaat

MiddleName

-

Affiliation

Department of Pathology, Faculty of Medicine, Alexandria University

Email

-

City

-

Orcid

-

First Name

Afaf

Last Name

El Sawa

MiddleName

-

Affiliation

Department of Oral Biology, Faculty of Dentistry, Alexandria Universit

Email

-

City

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Orcid

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First Name

hanan

Last Name

Ismail

MiddleName

-

Affiliation

Department of Orthodontics, Faculty of Dentistry, Alexandria University.

Email

-

City

-

Orcid

-

First Name

Abbas

Last Name

Zaher

MiddleName

-

Affiliation

Department of Orthodontics, Faculty of Dentistry, Alexandria University.

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-

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-

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Volume

49

Article Issue

June 2016

Related Issue

11753

Issue Date

2016-06-01

Receive Date

2016-01-27

Publish Date

2016-06-01

Page Start

1

Page End

10

Print ISSN

1110-435X

Online ISSN

2812-5258

Article File

EOS_Volume 49_Issue June 2016_Pages 1-10.pdf

PDF . 602.7kB

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https://eos.journals.ekb.eg/article_78681.html

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https://eos.journals.ekb.eg/service?article_code=78681

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Original Article

Type Code

1,120

Publication Type

Journal

Publication Title

Egyptian Orthodontic Journal

Publication Link

https://eos.journals.ekb.eg/

MainTitle

Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3

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Article

Created At

22 Jan 2023