An experimental investigation has been carried out to study the effect of the digestion temperature and the type of organic waste on the biogas production. The experimental work is performed by using three similar lab-scale batch-fed 240 liter biogas reactors. Each of the bioreactors is designed to operate under thermophilic (55 ± 2 ºC), mesophilic (40 ± 2 ºC) and ambient digestion modes during the same retention time of 31 days. Three experimental test runs have been carried out on three different mixtures of organic wastes including pure cattle manure, cattle manure mixed with rice straw and cattle manure mixed with hen manure. Daily measurements are recorded for the generated biogas volumes, pH values, average gas pressure and the ambient temperature during each test run. The results indicate that, total biogas yield in thermophilic mode gives an increase over mesophilic mode by 4%, 6.5% and 80% for the first, the second and the third run, respectively. Also, the total biogas production for ambient mode is lower than thermophilic mode by 72%, 80% and 60% for the first, second and third run, respectively. The maximum daily biogas yield was obtained at pH values in the range of (8.1 – 8.51) and (7.11 – 8.24) for thermophilic and both of mesophilic and ambient modes, respectively. In addition, it was observed that the maximum biogas yield has been achieved at a value of 9.4% of total solid content in the slurry.