Background: Chronic wound healing is a significant health
problem. Increased angiogenesis and blood flow had been
shown to facilitate the healing of chronic wounds. Negative
pressure wound therapy and platelet rich plasma may be used
for treatment of various chronic wounds, especially when
standard conventional therapy is not good enough or for
preparation for definitive closure.
Objectives: To compare the efficacy of negative pressure
wound therapy and platelet rich plasma in neovascularization
of chronic wound healing.
Patient and Method: Twenty patients with chronic wound
were divided into two groups. Wound tissue biopsies were
collected from the centeraland peripheral of the wound before
starting our procedure and after one, two and three weeks in
both groups. Evaluation of the wound clinically and Immunohistochemically
for vascular endothelial growth factor
(VEGF) and CD31 (cluster of differentiation) were done.
Results: PRP and NPWT were effective in neovasculization
and stimulation of healing process. The expression of VEGF
in PRP patients after the third week was mild 10%, moderate
20%, strong 70% and was mild 10%, moderate 20%, strong
70% in peripheral and central tissue biopsy respectively. While
in NPWT patient was mild 20%, moderate 30%, strong 50%
in both peripheral and central tissue biopsy. The average of
blood vessels formation in CD-31 was 9.95±3.64 after 3 week
in peripheral tissue biopsy and was 8.58±3.51 in central tissue
biopsy in PRP patients. While in NPWT patients was 8.35±3.25
in peripheraltissue biopsy and was 8.38±3.12 in central tissue
biopsy.
Conclusion: PRP and NPWT are effective in treatment
chronic wounds. PRP is more simple, less costly, shorter time
for the method, less painful, no hospital stay and less transmission
of infections.