Gut microbiota and dietary modifications get much more attention worldwide within the recent two decades. Therefore, the present study was carried out to evaluate the effects of dried supplementations of different percentage of sage (1, 2, 4 and 5%) on diabetic rats' healthy stats, intestinal microbiota composition and activities. Five diabetic groups of rat were used in this study, in addition to one group used as negative control group (healthy diet). All rats were used for determining body weight gain (BWG), glucose, cholesterol, high density lipoprotein-cholesterol (HDL-c), and low density lipoprotein-cholesterol (LDL-c). Additionally, intestinal microbioma have been measured including Bifidobacteria, Clostridium histolyticum, and Lactobacillus. The presented data illustrated that glucose levels were significantly (P≤0.05) decreased amongst sage treatment diabetic rats comparing to the control (+) group in independence manner for the first three concentrations (1, 2 and 4%). Then group fed 5% sage was slightly similar to 4% group. Also, the best colonic microbiota compositions were consequently matched in a dose independence manner with both Bifidobacteria, and Lactobacillus (probiotics species) comparing to Clostridium levels. Finally, cholesterol and LDL-c levels decreased in sage treatment groups comparing to control (+) group. In conclusion, consuming sage plays an important role in improving and showing potential effects on diabetic rat's health including enhancement of glucose, colonic microbiota and serum lipids profiles levels. So, sage could be used successfully as a suggestive of prebiotics dietary sources.