The present work was carried out to investigate the effect of some food additives (turnip leafs and roots, grape leafs and carrot roots) on enhancement colour of koi fish, Cyprinus carpio. Fish were divided into 8 groups in 16 glass aquaria (100 x 30 x 40 cm) with stocking density of 10 fish / aquarium (2 replicate aquaria were assigned for each experimental group). Fish were fed on plain food as a control (C), plain diet+ 5% turnip (T1), plain diet+ 5% grape (T2), plain diet+ 5% carrot (T3), plain diet+ 5% grape + carrot (T4), plain diet+ 5% grape + turnip (T5), plain diet+ 5% carrot + turnip (T6) and plain diet+ 5% turnip + grape + carrot (T7) over 4 months.
Results showed that, the highest amount of the red color pixels was recorded in the treated fish group T7 (fed on the 3 mixed food additives) at 30, 60, 90 and 120 days; followed by that of T6 (carrot + turnip) at 60 and 90 days and in T4 (grape +carrot) at 90 and 120 days.
Results also revealed that, the highest amount of the yellow color pixels was recorded at 90 and 120 days in the treated fish group T3 (carrot). But, the highest amount of the yellow color pixels was recorded in T4 (grape +carrot) at 30 and 60 days; followed by that of T6 (carrot + turnip) at 60, 90 and 120 days.
Results showed that, the highest amount of total color pixels was recorded in T3 (carrot) at 60, 90 and 120 days; followed by that of T6 (carrot + turnip) and in T4 (grape +carrot) at 60, 90 and 120 days.
In conclusion the best enhancement of red color in koi fish, C. carpio was recorded in the fish fed on the diet containing 5% of the mixture of all experimental additives (carrot roots+ grape leafs+ turnip leafs and roots). The enhancement of yellow color was recorded in fish fed on the diet containing 5% “carrot roots" for more than 30 days. The enhancement of total color of koi fish, C. carpio was recorded in fish fed on the diet containing 5% “carrot roots, or carrot roots with turnip leafs and roots".