The catalytic properties of alumina (ɣ-Al₂O₃), Graphene Oxide (GO) and Graphene Oxide –alumina was studied in transesterification of sunflower oil to biodiesel. The catalytic tests were carried out at standard reaction conditions; reaction temperature 60˚C, methanol to oil ratio 12:1, catalyst to oil ratio 1:100 and reaction time 1 hr.In the case of graphene oxide samples their performances in conversion of oil to biodiesel depends on the method of preparation. On the other hand, the catalytic activity results showed that addition of graphene oxide to alumina significantly improved its transesterification reaction where, conversion increased from 13% for neat alumina and 18% for GO to 28% for GO-alumina. This effect was attributed to that GO-alumina had higher amounts of acidic sites than its parent materials.