Hematological reference values are essential for the interpretation of the Results of laboratory tests and treatment decisions. The full blood count reference intervals in Egypt are generally derived from non-Egyptian subjects. This study aimed to establish hematological reference intervals for healthy adults in Egypt.  From January 2018 to January 2020, 5620 volunteers were recruited for this study. A total of 5620 was divided into 1846 (32.8 %) males and 2926 (52.1 %) Females. but 848 (15.1 %) volunteers were excluded because of positive laboratory screening results. Males had a higher median of hemoglobin (14 g/dL versus 12.3 g/dL), median of RBC (4.96 × 10⁶/ µL versus 4.35 × 10⁶/ µL), and hematocrit (41% versus 36.2%) than females. The difference in hemoglobin, RBC, and hematocrit by gender was statistically significantly different (p < 0.05). Males had lower median white blood cells (6.7× 10³/ µL versus 6.9× 10³/ µL) and median of Platelets (230 × 10³/ µL versus 252.5 × 10³/ µL) than Females (p < 0.05). in comparison to reference values that are commonly used in Egypt, the hemoglobin levels, RBC, and hematocrit from this study were lower.