Reaction time (RT) is an important method for studying the centralized data processing speed of individuals and the coordinated and rapid response to their movement. Accordingly, RT is essential for officers, soldiers, doctors, drivers, pilots, security guards, etc. to succeed in their fields. Previous studies have mainly focused on RT with regard to the psychological and neurological domain, and there have been very few studies on reaction time and nutritional support beside quality of food or drink consumed. The current study aimed to improve the reaction time of healthy college students after providing them with natural nutritional support highly rich with choline, zinc, and vitamin B₁₂ represented in the food source “municipal liver" by 250 grams after cooking. The study sample consisted of 14 students from the Faculty of Agricultural and Environmental Sciences at Suez Canal University. The students were divided into two groups, the control group of 5 students and the experimental group of 9 students. A blood sample was taken from all students before starting the experiment to measure the proportion of choline, zinc, and vitamin B₁₂ in their blood plasma, and the reaction time was recorded before using five measures of computer reaction time on the Internet according to Jim Allen (2002). Daily nutritional support was given to the students of group (2) using the natural food source, the municipal liver, for 30 days. After 30 days, a post-laboratory blood sample was drawn to measure the proportion of choline, zinc, and vitamin B₁₂ in the investigated students' blood plasma of both groups. Also, the post-reaction time was recorded for them. The findings revealed that there was a significant improvement in RT for students of a group (2) who took natural supplements compared to the control group. The results also indicated that their research sample performed better in laboratory analyses (P < 0.01) and response time schedules Online action (P < 0.01). According to the findings of the research, choline, zinc, and vitamin B₁₂ as natural food sources have a positive effect on the improvement of reaction time.