The present study aims to evaluate the effects of seminal Reactive oxygen species (ROS), lipids peroxidation, and total antioxidant capacity (TAC) on the traditional and functional semen characteristic analysis and quality intracytoplasmic sperm injection (ICSI) outcomes. The study group demonstrated that the elevated levels of seminal ROS and lipids peroxidation greatly reduced sperm concentration, total sperm count, total motility, normal morphology, and seminal TAC and increased seminal leukocytes count compared to control group. In the study group, the elevated levels of seminal ROS and lipids peroxidation markedly attenuated fertilization, cleavage, and blastocysts formation rates and decreased D3 and D5 top quality embryos development grade compared to control group. Finally, the study results indicated that administration of antioxidant supplementation can improve sperm characteristic parameters and quality ICSI outcomes, decrease levels of seminal ROS and lipids peroxidation, and increase its TAC level.