The emergence of extreme drug resistant (XDR) Pseudomonas aeruginosa  represents a major problem in health care settings. This work aimed to study the prevalence of some OXA genes and biofilm formation among P. aeruginosa clinical isolates collected from Intensive Care Units (ICUs), Ain-Shams University, Cairo, Egypt. Among 70 Gram negative clinical isolates, 25 isolates were phenotypically identified as P. aeruginosa. Antibiotic sensitivity and minimum inhibitory concentration (MIC) for 18 different antibiotics (Ticarcillin, Ticarcillin /Clavulanic acid, Piperacillin, Piperacillin-Tazobactam, Ceftazidime, Cefepime, Meropenem, Amikacin, Gentamicin, Aztreonam, Imipenem, Meropenem, Tobramycin, Ciprofloxacin, Plefloxacin, Minocyclin, Rifampcin, Trimethothoprim/ Sulfamethoxazole and colistin) were carried out against P. aeruginosa isolates using VITEK 2.  All isolates were resistant to all antibiotics except colistin that only 12% were resistant. Real time polymerase chain reaction with specific primers was used to detect the presence of selected OXA genes. OXA group I and OXA group II were detected in 44 and 52%, respectively, while OXA group III, OXA51, OXA23, OXA 24 and OXA 58 were totally absent. Biofilm formation assay showed strong, weak and moderate biofilm formation in 44, 28 and 12% of the isolates, respectively, while 16% were non biofilm forming isolates.