Beta
ManageSearchChatDiscover
230601

Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Hepatocellular carcinoma (HCC) is the tertiary greatest communal malignant cancer that origins mortality globally. A high prevalence of return means that even while cancer is getting more treatable in its early stages, advanced cases have a bad prognosis. To properly treat HCC, it is now necessary to understand its pathogenic process and its associated genetic abnormalities. The next-generation sequencing (NGS) was used to discover PIK3CA somatic mutations in HCC Egyptian patients. In the present study a unique NGS panel (AmpliSeq) containing the PIK3CA gene was utilized to examine 21 liquid biopsy samples from patients with HCC. The results indicated that over 40 -single-nucleotide-variation (SNV) were recognized in PIK3CA gene with incidence of 85.7%. The changes were dispersed between deleterious, undefined significance, and tolerated deviations, where the preponderance of the changes were the missense variants (68%),synonymous(25%), and (7%) for stop-gained. It was concluded from this research that detection of numerous somatic mutations of PIK3CA can assist in the etiology of HCC.

DOI

10.21608/ajbs.2022.230601

Keywords

PIK3CA, HCC, Non-Synonymous, Mutation, SNV, NGS

Authors

First Name

Amr

Last Name

Shugaa Addin

MiddleName

M.

Affiliation

Molecular diagnostics and therapeutics department,GEBRI.University of Sadat city

Email

shogaa2002@yahoo.com

City

Cairo

Orcid

0000-0002-3579-8409

First Name

Randa

Last Name

Talaat

MiddleName

M

Affiliation

Children Cancer Hospital 57357, Egypt

Email

randa_talaat@hotmail.com

City

Caro

Orcid

-

First Name

Moustafa

Last Name

Sakr

MiddleName

A

Affiliation

Department of Molecular Diagnostic and Therapeutic, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Egypt

Email

dr_sakr2000@yahoo.com

City

Cairo

Orcid

-

First Name

Mohamed

Last Name

Khalifa

MiddleName

K

Affiliation

Children Cancer Hospital 57357, Egypt

Email

mohamed.kamal@omicsense.com

City

Cairo

Orcid

-

First Name

Ehab

Last Name

Ahmed

MiddleName

A

Affiliation

Chemistry department, Faculty of science, Cairo University, Egypt.

Email

ehab2003@yahoo.com

City

Cairo

Orcid

-

First Name

Ghada

Last Name

Nasr

MiddleName

M

Affiliation

Department of Molecular Diagnostic and Therapeutic, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Egypt

Email

nasr_mi@yahoo.com

City

Cairo

Orcid

-

First Name

Manal

Last Name

El Hamshary

MiddleName

O

Affiliation

Department of Molecular Diagnostic and Therapeutic, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Egypt

Email

manshry64@gmail.com

City

Cairo

Orcid

-

Volume

18

Article Issue

1

Related Issue

32997

Issue Date

2022-06-01

Receive Date

2022-03-17

Publish Date

2022-06-01

Page Start

29

Page End

38

Print ISSN

1687-4870

Online ISSN

2314-5501

Article File

AJBS_Volume 18_Issue 1_Pages 29-38.pdf

PDF . 892.9kB

Link

https://ajbs.journals.ekb.eg/article_230601.html

Detail API

https://ajbs.journals.ekb.eg/service?article_code=230601

Order

3

Type

Original Article

Type Code

1,101

Publication Type

Journal

Publication Title

African Journal of Biological Sciences

Publication Link

https://ajbs.journals.ekb.eg/

MainTitle

Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians

Details

Type

Article

Created At

22 Jan 2023