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230600

Mutagenic effect of acridine orange on the production of EPS in Bacillus sp., its applications as anticoagulant and genetic diversity by ISSR and SCoT technique

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Last updated: 25 Dec 2024

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Abstract

Many important substances are produced by some organisms, such as extracellular polysaccharides (EPS). The genus Bacillus is an important one in this field. As a result, in this study, a defined strain of Bacillus sp < /em>. BAB3450 (Gen Bank) was used to produce EPS with the goal of increasing productivity by introducing acridine orange mutations (AO). The results were obtained from three mutations that were more productive than the wild type. On the other hand, using primers Inter-Simple Sequence Repeat (ISSR) and Start Codon Targeted Polymorphism (SCoT), a comparison with the three mutants and wild type were made at the molecular level. Eleven ISSR primers were used. The results showed that ISSR-19, with 92.86% polymorphism, was the best primer. Using ten SCoT primers, the results showed that SCoT-2 was the best primer, with 90% polymorphism. For the ISSR primer and SCoT primers, the wild type and three mutants were divided into two major groups based on marker analysis with distance 60 to 75% and 55 to 65%, respectively. Cluster analyses were used to create a dendrogram of Bacillus strains and mutants, revealing high genetic variations between the wild type and mutants. Finally, extracellular polysaccharides (EPS) have a wide range of industrial applications in biomedicine, such as anticoagulant. The EPS from wild type and three mutants were tested for anticoagulant comparing with heparin. Mutant No.1 gave the best anticoagulant (15˃%), while the wild type, mutant No.2, mutant No.3 and mutant No.4 gave 10˃% comparing with heparin (80˃%). 

DOI

10.21608/ajbs.2022.230600

Keywords

Genetic diversity, Anticoagulant, Bacillus, Acridine orange, ISSR and SCoT

Authors

First Name

Hossam

Last Name

EL-Shaer

MiddleName

F.A.

Affiliation

Botany Department (Genetics), Faculty of Agriculture, Al-Azhar Univ., Cairo, Egypt

Email

hosamelshaer805@azahar.edue.eg

City

Cairo

Orcid

-

Volume

18

Article Issue

1

Related Issue

32997

Issue Date

2022-06-01

Receive Date

2022-03-17

Publish Date

2022-06-01

Page Start

15

Page End

27

Print ISSN

1687-4870

Online ISSN

2314-5501

Article File

AJBS_Volume 18_Issue 1_Pages 15-27.pdf

PDF . 694.7kB

Link

https://ajbs.journals.ekb.eg/article_230600.html

Detail API

https://ajbs.journals.ekb.eg/service?article_code=230600

Order

2

Type

Original Article

Type Code

1,101

Publication Type

Journal

Publication Title

African Journal of Biological Sciences

Publication Link

https://ajbs.journals.ekb.eg/

MainTitle

Mutagenic effect of acridine orange on the production of EPS in Bacillus sp., its applications as anticoagulant and genetic diversity by ISSR and SCoT technique

Details

Type

Article

Created At

22 Jan 2023