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153144

Histological studies on somatic embryogenesis in rice (Oryza sativ L.)

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Last updated: 04 Jan 2025

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Abstract

Rice (Oryza sativa) embryogenic calli were obtained from mature zygotic embryos culture using two varieties of rice, Sakha 101 and Sakha104 on Murashige and Skoog (1962 ) medium supplemented with 1mg/l, 2mg/l and 3mg/l 2,4- dichlorophenoxy acetic acid. The results indicated that the best medium was that containing 3mg/l. Histological analysis of somatic embryogenesis revealed that after two weeks of culture of explants on the callus induction medium, somatic embryo development began with a cluster of proembryogenic cells in the peripheral region of the calli. The outer cell layer of embryogenic calli consisted of small and isodiametric cells, each  with a dense cytoplasm and a prominent nucleus and nucleolus, whereas the inner cell layer is composed of large cells with small nuclei and large vacuoles. These embryogenic cells underwent a series of organized divisions and formed the proembryo with a well-defined protodermis. At 20 days after initiation of culture, these proembryos continued a series of organized divisions and gave rise to globular somatic embryos which are delimited by a well-defined layer of epidermal cells with conspicuous nuclei. These somatic embryos had no apparent vascular connection with the mother tissue and had a suspensor. After ten days of culture on regeneration medium, globular somatic embryos developed into heart-shaped somatic embryos, which consisted of cells with prominent nuclei and dense cytoplasm. After 15 days of culture on regeneration medium torpedo-shaped somatic embryos were observed. Then, after 25 to30 days, it turned into a monocotyledon form.
 

DOI

10.21608/ajbs.2021.153144

Keywords

Oryza sativa, rice, In vitro culture, histology, somatic embryogenesis, morphogenesis

Authors

First Name

hassan

Last Name

mesbah

MiddleName

abdallah

Affiliation

Botanty. Dept. (Genetics) Fac.of Agric., El-azhar Univ., Cairo, Egypt

Email

hassan.elsayed85@azhar.edu.eg

City

-

Orcid

-

First Name

Abd El-Salam

Last Name

Nassar

MiddleName

M.

Affiliation

Botanty Dept. (Genetics), Fac.of Agric., El-azhar Univ., Cairo, Egypt

Email

-

City

Cairo

Orcid

-

First Name

Medhat

Last Name

El-Sheikh

MiddleName

A.

Affiliation

Botanty Dept. (Genetics), Fac.of Agric., El-azhar Univ., Cairo, Egypt

Email

-

City

Cairo

Orcid

-

Volume

17

Article Issue

1

Related Issue

21458

Issue Date

2021-12-01

Receive Date

2021-02-13

Publish Date

2021-12-01

Page Start

73

Page End

81

Print ISSN

1687-4870

Online ISSN

2314-5501

Link

https://ajbs.journals.ekb.eg/article_153144.html

Detail API

https://ajbs.journals.ekb.eg/service?article_code=153144

Order

7

Type

Original Article

Type Code

1,101

Publication Type

Journal

Publication Title

African Journal of Biological Sciences

Publication Link

https://ajbs.journals.ekb.eg/

MainTitle

Histological studies on somatic embryogenesis in rice (Oryza sativ L.)

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Type

Article

Created At

22 Jan 2023