Nutrients nano-forms in livestock's feed are mainly intended to increase the production performance, enhance immunity and antioxidant activities. The current experiment was undertaken by feeding 150 (divided to five group control, N-acetyl cysteine (NAC), NAC chit nano-composite (different levels (30, 60, 120 μg / kg diet)), one day old broiler chicks for 42 days. The effect of these additives as antioxidant on liver and kidney function tests of broiler chicks were investigated. The results were compared with those obtained from feeding another group of broiler chicks on the normal diet but after addition of commercial antioxidant, NAC (5.2g/kg feed). The results showed that chicken in the groups fed on (60,120 μg / kg feed) of NAC chit revealed significant (P< 0.05) improved antioxidant status, liver and kidney function indices as compared with control and the other dietary treated groups. Structure of mitochondria in HRTEM examination showed slight to moderate swelling due to increase of NAC chit concentration and found NAC chit appeared inside the mitochondria.